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dulbecco s phosphate buffered saline dpbs  (Thermo Fisher)


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    Structured Review

    Thermo Fisher dulbecco s phosphate buffered saline dpbs
    Dulbecco S Phosphate Buffered Saline Dpbs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/1x+phosphate+buffered+saline/Phosphate-buffered+saline+(DPBS%2C+1X)%2C+Dulbecco's+formula/pmc13241941-84-15-24
    Average 99 stars, based on 1 article reviews
    dulbecco s phosphate buffered saline dpbs - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Incubation:

    Article Title: Ectromelia virus growth factor is not secreted from infected cells and plays no apparent role for virus replication in vitro.
    Article Snippet: Many poxviruses of vertebrates express proteins related to epidermal growth factor (EGF).. We recently reported that recombinant ectromelia virus growth factor (ECGF) acts as a bona fide mitogen when added to cultured cells.. Additionally, while most GF proteins from other related orthopoxviruses contain a transmembrane domain, ECGF lacks this region and, as such, should be directly released without the need for proteolytic processing at the plasma membrane.

    Saline:

    Article Title: Ectromelia virus growth factor is not secreted from infected cells and plays no apparent role for virus replication in vitro.
    Article Snippet: Many poxviruses of vertebrates express proteins related to epidermal growth factor (EGF).. We recently reported that recombinant ectromelia virus growth factor (ECGF) acts as a bona fide mitogen when added to cultured cells.. Additionally, while most GF proteins from other related orthopoxviruses contain a transmembrane domain, ECGF lacks this region and, as such, should be directly released without the need for proteolytic processing at the plasma membrane.

    Article Title: Kinemomics: spatiotemporal morphodynamic mapping of ventricular kinematic subpopulations in organotypic fetal heart slices
    Article Snippet: .. After live imaging, heart slice sections were fixed with 4% PFA overnight at 4°C, washed with 1x phosphate-buffered saline (PBS; Gibco, 20012-027) twice for at least 1 hr, placed in 15% sucrose (Thermo Scientific, A15583.36) in PBS at 4°C until sinks, then transferred into 30% sucrose in PBS until sinks. ..

    Article Title: Butyrate synergizes with glucose to promote anaerobic growth of Staphylococcus aureus via anaplerotic metabolism and stress response pathways
    Article Snippet: Following incubation, the plates were removed from the anaerobic chamber, and the exterior surfaces were disinfected with 70 % ethanol (Decon Labs). .. The supernatant was carefully removed by pipetting, and the adhered biofilms were gently washed with 0.2 mL of 1X phosphate-buffered saline (PBS; Gibco). .. Biofilms were stained with 0.2 mL of 0.1 % crystal violet (Sigma Aldrich) for 10 min. After staining, the crystal violet was aspirated, and the stained biofilms were washed twice with 0.2 mL of sterile 1X PBS.

    Article Title: AdMSC spheroids encapsulating antioxidant hybrid protein carrier for irradiation-damaged salivary gland repair
    Article Snippet: To evaluate changes in secondary protein structure, CD spectra were acquired using a circular dichroism spectropolarimeter (J-815; Jasco, Japan). .. Gel and GG were each prepared at a concentration of 0.1 mg mL −1 in 1X phosphate-buffered saline (PBS, pH 7.4, Thermo Fisher Scientific). ..

    Article Title: Gel-free library preparation for next-generation RNA sequencing and small RNA quantification.
    Article Snippet: Upon entry into the late-exponential phase, as indicated by optical density (OD) 600 values between 0.6-0.7, the bacterial culture was aliquoted (1 mL) into sterile Eppendorf tubes. .. Bacterial cell pellets were harvested by centrifugation at 500 × g and 4 °C for 5 min and subsequently washed by resuspension in 1 mL of 1X phosphate buffered saline (PBS; pH = 7.4; Thermo Fisher Scientific), followed by centrifugation using the same settings. .. Isolation of small RNAs from the bacterial cells was conducted following the user guide of the PureLink miRNA Isolation Kit (Thermo Fisher Scientific) with modifications.

    Article Title: Collagen II hydrogel-mediated sustained delivery of lacosamide attenuates cartilage degeneration and pain in osteoarthritis
    Article Snippet: .. Their initial weight (W 0 ), was recorded, and they were then immersed in 1x phosphate-buffered saline (PBS, Gibco) at a constant temperature of 37 °C. ..

    Article Title: Substrate and target selectivity of 4′-fluoroadenosine against viral and host polymerases
    Article Snippet: .. Following inactivation by formalin, plates were removed from the BSL-4 laboratory and washed in 1x phosphate-buffered saline (PBS, Thermo Fisher Scientific) permeabilized with 0.1% (v/v) Triton X (Acros Organics) for 15 min and blocked with 3.5% bovine serum albumin (BSA, 1 h). .. Cells were stained with mouse anti-EBOV glycoprotein (4F3, IBT Bioservices) for 3 h at room temperature.

    Article Title: Histotripsy treatment reduces tumor burden and extends survival in an orthotopic mouse model of osteosarcoma
    Article Snippet: To prepare for tumor induction, cells were lifted with 1.25% trypsin and washed in 1X sterile phosphate buffered saline (PBS, Gibco). .. Cells were resuspended in a 50% 1X phosphate buffered saline (PBS, Gibco):Matrigel® (Corning Inc., Corning, NY, USA) solution, then 50 uL of cell suspension containing 1x10 6 cells was injected into the right hindlimb via para-tibial injection ( , , ). .. Mice were anesthetized briefly for tumor cell injection via inhaled isoflurane (2.0 L/min oxygen and 2% isoflurane) and a low-flow vaporizer (SomnoFlo®, Kent Scientific Corporation, Torrington, CT, USA).

    Imaging:

    Article Title: Kinemomics: spatiotemporal morphodynamic mapping of ventricular kinematic subpopulations in organotypic fetal heart slices
    Article Snippet: .. After live imaging, heart slice sections were fixed with 4% PFA overnight at 4°C, washed with 1x phosphate-buffered saline (PBS; Gibco, 20012-027) twice for at least 1 hr, placed in 15% sucrose (Thermo Scientific, A15583.36) in PBS at 4°C until sinks, then transferred into 30% sucrose in PBS until sinks. ..

    Concentration Assay:

    Article Title: AdMSC spheroids encapsulating antioxidant hybrid protein carrier for irradiation-damaged salivary gland repair
    Article Snippet: To evaluate changes in secondary protein structure, CD spectra were acquired using a circular dichroism spectropolarimeter (J-815; Jasco, Japan). .. Gel and GG were each prepared at a concentration of 0.1 mg mL −1 in 1X phosphate-buffered saline (PBS, pH 7.4, Thermo Fisher Scientific). ..

    Centrifugation:

    Article Title: Gel-free library preparation for next-generation RNA sequencing and small RNA quantification.
    Article Snippet: Upon entry into the late-exponential phase, as indicated by optical density (OD) 600 values between 0.6-0.7, the bacterial culture was aliquoted (1 mL) into sterile Eppendorf tubes. .. Bacterial cell pellets were harvested by centrifugation at 500 × g and 4 °C for 5 min and subsequently washed by resuspension in 1 mL of 1X phosphate buffered saline (PBS; pH = 7.4; Thermo Fisher Scientific), followed by centrifugation using the same settings. .. Isolation of small RNAs from the bacterial cells was conducted following the user guide of the PureLink miRNA Isolation Kit (Thermo Fisher Scientific) with modifications.

    Suspension:

    Article Title: Histotripsy treatment reduces tumor burden and extends survival in an orthotopic mouse model of osteosarcoma
    Article Snippet: To prepare for tumor induction, cells were lifted with 1.25% trypsin and washed in 1X sterile phosphate buffered saline (PBS, Gibco). .. Cells were resuspended in a 50% 1X phosphate buffered saline (PBS, Gibco):Matrigel® (Corning Inc., Corning, NY, USA) solution, then 50 uL of cell suspension containing 1x10 6 cells was injected into the right hindlimb via para-tibial injection ( , , ). .. Mice were anesthetized briefly for tumor cell injection via inhaled isoflurane (2.0 L/min oxygen and 2% isoflurane) and a low-flow vaporizer (SomnoFlo®, Kent Scientific Corporation, Torrington, CT, USA).

    Injection:

    Article Title: Histotripsy treatment reduces tumor burden and extends survival in an orthotopic mouse model of osteosarcoma
    Article Snippet: To prepare for tumor induction, cells were lifted with 1.25% trypsin and washed in 1X sterile phosphate buffered saline (PBS, Gibco). .. Cells were resuspended in a 50% 1X phosphate buffered saline (PBS, Gibco):Matrigel® (Corning Inc., Corning, NY, USA) solution, then 50 uL of cell suspension containing 1x10 6 cells was injected into the right hindlimb via para-tibial injection ( , , ). .. Mice were anesthetized briefly for tumor cell injection via inhaled isoflurane (2.0 L/min oxygen and 2% isoflurane) and a low-flow vaporizer (SomnoFlo®, Kent Scientific Corporation, Torrington, CT, USA).



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